The Handbook of Plant Metabolomics

The Handbook of Plant Metabolomics

Language: English

Pages: 448

ISBN: 3527327770

Format: PDF / Kindle (mobi) / ePub

This is the newest title in the successful Molecular Plant Biology Handbook Series. Just like the other titles in the series this new book presents an excellent overview of different approaches and techniques in Metabolomics. Contributors are either from ivy-league research institutions or from companies developing new technologies in this dynamic and fast-growing field. With its approach to introduce current techniques in plant metabolomics to a wider audience and with many labs and companies considering to introduce metabolomics for their research, the title meets a growing market. The Kahl books are in addition a trusted brand for the plant science community and have always sold above expectations.

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Molecular Plant Biology Handbook Series Günter Kahl is Professor for Plant Molecular Biology at the Biocenter of Johann Wolfgang Goethe-University of Frankfurt/Main, Germany. After gaining his PhD in plant biochemistry, he spent two postdoctoral years at Michigan State University, East Lansing, USA, joining Professor Joe Varner, and at the California Institute of Technology, Pasadena, California, with Professor James Bonner. His main research interests are: Sequencing and analysis of fungal,

Liang, Y.-S., Choi, Y.H., Kim, H.K., Linthorst, H.J.M., and Verpoorte, R. (2006) Metabolomic analysis of methyl jasmonate treated Brassica rapa leaves by 2-dimensional NMR spectroscopy. Phytochemistry, 67, 2503–2511. Mirnezhad, M., Romero-Gonzalez, R.R., Leiss, K.A., Choi, Y.H., Verpoorte, R., and Klinkhamer, P.G.L. (2010) Metabolomic analysis of host plant resistance to thrips in wild and cultivated tomatoes. Phytochem. Anal., 21, 110–117. Choi, Y.H., Tapias, E.C., Kim, H.K., Lefeber, A.W.,

and Protocols Figure 4.4 Total ion current (TIC) chromatogram of an avocado extract using GC Â GC–TOFMS. A tissue sample of 40 mg was homogenized and extracted using methanol. The extract was dried, derivatized, and analyzed by GC Â GC–TOFMS. Major metabolites are labeled, extraction surrogates are italicized, and derivatization standards are printed in black. 4.2 Methods and Protocols 4.2.1 Instrumentation  A LECO (St. Joseph, MI, USA) Pegasus 4D GC Â GC–TOF-MS system comprising an Agilent

Interactions 4) Place the prepared leaves upside down on the perlite layer of boxes-A (two leaves per six plants). 5) Rebuild the setup. 6) After a defined time interval (e.g., 3 days) of inoculation, remove the leaves. 7.2.3 Sampling and Quenching of Plant Tissue (Roots and Leaves) The typical mass of a root tip is a few milligrams. Thus, around 50 root tips have to be collected for GC–MS measurements. In the case of leaves, which weigh around 3–4 g, a single leaf offers enough material for >10

and Shyur, L.F. (2007) A galactolipid possesses novel cancer chemopreventive effects by suppressing inflammatory mediators and mouse B16 melanoma. Cancer Res., 67, 6907–6915. Chiang, Y.M., Chuang, D.Y., Wang, S.Y., Kuo, Y.H., Tsai, P.W., and Shyur, L.F. (2004) Metabolite profiling and chemopreventive bioactivity of plant extracts from Bidens pilosa. J. Ethnopharmacol., 95, 409–419. James, A.T. and Martin, A.J. (1952) Gas– liquid partition chromatography: the separation and micro-estimation of

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