Laboratory Manual for Non-Majors Biology

Laboratory Manual for Non-Majors Biology

James W. Perry, Joy B. Perry, David A. Morton

Language: English

Pages: 750

ISBN: 0840053800

Format: PDF / Kindle (mobi) / ePub

About the book:

Through hands-on lab experience, this biology laboratory manual reinforces biology concepts to help you get a better grade. Exercises, pre-lab questions, and post-lab questions enhance your understanding and make lab assignments easy to complete and easy to comprehend.

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Figure 3-9  How to make a wet mount. Photo by J. W. Perry. 4. Slowly lower the coverslip with a dissecting needle so as not to trap air bubbles (Figure 3-9b). 5. Observe the wet mount, first at low magnification and then with higher power. Air may be trapped either in the cork or as free bubbles (Figure 3-10). Trapped air will appear dark and refractive around its edges. This effect is caused by sharply bending rays of light. Draw what you see in Figure 3-11. Note the total magnification

correlation; 6. describe the design of a typical research article in biology; 7. do a bioassay as an example of an experiment. Introduction To appreciate biology or, for that matter, any body of scientific knowledge, you need to understand how the scientific method is used to gather that knowledge. We use the scientific method to test the predictions of possible answers to questions about nature in ways that we can duplicate or verify. Answers supported by test results are added to the

timing ■■ PROCEDURE 1. Designate at least one exerciser, a data recorder, an exercise timer, and a setup Caution manager from your team. Do not inhale! When 2. Label the three small beakers “Trial 1,” “Trial 2,” and “Trial 3.” the solution changes 3. Measure and pour 10 mL of BTB solution in each beaker. color to green or ­yellow, 4. At time 0, the exerciser slowly blows through a straw into the bottom of record the elapsed Trial 1 beaker. time (in seconds) in 5. Repeat the

Suppose the long pair of homologous ­chromosomes codes for two traits, skin pigmentation and the presence of attached earlobes in humans. We’ll let the capital letter A represent the allele for normal pigmentation and a lowercase a the allele for ­albinism (the absence of skin pigmentation); F will represent free earlobes and f attached earlobes. A ­suggested marking sequence is illustrated in Figure 12-7. 5. Let’s assign a gene to our second homologous pair of chromosomes, the short pair.

layers. Nucleic acids precipitate at the boundary between alcohol and water. The nucleic acids you have extracted are not pure; they contain cellular debris as well as adhering proteins. You will test your extracted precipitate to identify its major component. 1. Use a 10-mL graduated cylinder to measure 3 mL of TBE buffer solution into a clean test tube. 2. Slowly twirl the skewer or loop to wrap up the ­precipitated material and withdraw the skewer from the test tube. 3. Place the

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